Custom Polyclonal Antibody Packages

This page lists a few of our many possible polyclonal antibody production packages.  To view our general philosophy on antibody production, please click here.

All descriptions below are listed as using rabbits by default. Click here for a price list of comparative available protocols in all species.

Standard Rabbit Package

All of our offerings are based on our Standard Package, which includes:
  • Antigen design/Bioinformatics
  • 5mg uncoupled peptide sent to customer, 4mg coupled to KLH, 1mg for ELISA
  • Three immunizations of two rabbits (New Zealand White - SPF)
  • 5mL pre-immune serum per rabbit
  • Two production bleeds (40-50mL antiserum) per rabbit
  • ELISA on 1st production bleed

Most of our customers also add affinity purification, which helps to remove potential background signals across multiple application types.

Affinity Purified Rabbit Package

  • Antigen design/Bioinformatics
  • ~15-mer peptide, 14mg at >85%
  • 5mg to customer, 4mg to KLH, 1mg for ELISA, 4mg affinity column
  • Three immunizations of two rabbits (New Zealand White - SPF)
  • 5mL pre-immune serum per rabbit
  • Two production bleeds (40-50mL antiserum) per rabbit
  • ELISA on 1st production bleed
  • Affinity purification of 40-50mL antiserum

If you have an existing antigen, we can immunize with this instead of a peptide.  To purify serum from these types of projects we typically recommend purifying by Protein A instead of affinity purification using your protein.  Standard linkage chemistries for this can occlude potential antibody epitopes within your protein, risking of loss of viable material in purification.

If you would rather immunize with a protein antigen but do not have existing material or a commercial source, email us at antibody@vivitide.com to initiate a conversation about how we can help.

Looking to increase your overall chances for success, but not double your budget?  We can co-immunize the same set of animals with multiple peptides at once.  Pricing will vary depending on the number of peptides you wish to combine, and the number and type of purifications that are performed.  See the pricing list for details.

Modification Specific Antibodies - Reagents You Can Trust

Post-translational modifications regulate a myriad of biological functions.  Studying and tracking these events with antibodies you can trust are a rock you can build the rest of your research on. We have made thousands of antibodies in this fold, using a variety of different types of modifications (phosphorylation, methylation, acetylation, cleavage-site, nitrosylation, ubiqitination, etc.).  The approach that works for all of these is straightforward and essentially the same: Immunize with the target modified peptide and perform purifications on the resultant sera using columns made with both the target peptide and a negative control peptide or peptides.  Different projects can require subtle adjustments to this process, of course, but this general procedure is the start of what we know ensures the most specific resultant end material possible.  Why settle for anything less?

Our Modification Specific Protocol Structure

  • Antigen design/Bioinformatics
  • Two peptides at >90% purity, 14mg modified, 10mg non-modified peptide
  • 5mg uncoupled to customer, 1mg for ELISA, 4mg column each peptide
  • 4mg modified-peptide coupled to KLH
  • Three immunizations of two rabbits (New Zealand White - SPF)
  • 5mL pre-immune serum per rabbit
  • Two production bleeds (40-50mL antiserum) per rabbit
  • ELISA on 1st production bleed (each peptide)
  • Double affinity purification of 40-50mL antiserum to yield modification-specific antibody
Questions? Do not hesitate to call us at 888-343-5974 or email us at us at sales@vivitide.com. Custom Monoclonal Antibody Production, Custom Peptide Synthesis, and other services are also available. View our services here. We also carry antibodies and antisera in our online shop.