CoV-2 S1 (319-541), Sf9

Product Name CoV-2 S1 (319-541), Sf9

Product Number SARS-049

Synonym(s) Coronavirus 2019-nCoV Spike Glycoprotein-S1 Receptor Binding Domain Recombinant,SF9; Severe acute respiratory syndrome coronavirus 2; COVID-19; COVID-19 virus; COVID19; HCoV-19; Human coronavirus 2019; SARS-2; SARS-CoV2; SARS2; SARS-CoV-2 SP RBD; 2019-nCoV SP RBD; 2019-nCoV; 2019-nCoV; Spike RBD Protein

One-Letter Code ADPRVQPTES IVRFPNITNL CPFGEVFNAT RFASVYAWNR KRISNCVADY SVLYNSASFS TFKCYGVSPT KLNDLCFTNV YADSFVIRGD EVRQIAPGQT GKIADYNYKL PDDFTGCVIA WNSNNLDSKV GGNYNYLYRL FRKSNLKPFE RDISTEIYQA GSTPCNGVEG FNCYFPLQSY GFQPTNGVGY QPYRVVVLSF ELLHAPATVC GPKKSTNLVK NKCVNFHHHH HH

Introduction A human infecting coronavirus (viral pneumonia) called 2019 novel coronavirus, 2019-nCoV was found in the fish market at the city of Wuhan, Hubei province of China on December 2019.The 2019-nCoV shares an 87% identity to the 2 bat-derived severe acute respiratory syndrome 2018 SARS-CoV-2 located in Zhoushan of eastern China. 2019-nCoV has an analogous receptor-BD-structure to that of 2018 SARS-CoV, even though there is a.a. diversity so thus the 2019-nCoV might bind to ACE2 receptor protein (angiotensin-converting enzyme 2) in humans.While bats are probably the host of 2019-nCoV, researchers suspect that animal from the ocean sold at the seafood market was an intermediate host. RSCU analysis proposes that the 2019-nCoV is a recombinant within the viral spike glycoprotein between the bat coronavirus and an unknown coronavirus.

Description Recombinant Coronavirus 2019-nCoV Spike Glycoprotein-S1 Receptor Binding Domain is a single, glycosylated polypeptide chain containing a total of 232 amino acids (319-541) and having a calculated M.W. of 26.2 kDa.

CoV-2 S1 (319-541) is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.

Source Sf9, Baculovirus cells.

Formulation
CoV-2 S1 (319-541) solution (0.25mg/ml) contains Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.

Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Biological Activity Measured by its binding ability in a functional ELISA with Human ACE-2 (CAT# enz-1159).

Physical Appearance Sterile filtered clear solution.


Purity 
Protein is >95% pure as determined SDS-PAGE.

Storage Conditions -20ºC

Delivery Details 7-10 days

Special Note(s) For research use ONLY. Not for use on humans.

Document(s) SDS