Product Name: EPO a Human

Product No: CYT-201

Synonym(s): Erythropoietin-Alpha Human Recombinant, Erythropoietin-Alpha, EPO-a, EPO-alpha, EP, MGC138142.

One-Letter-Code: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR.

Description: Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 36-40kDa. EPO-a is purified by proprietary chromatographic techniques.

Protein Derived From: Chinese Hamster Ovary Cells(CHO).

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Stability: Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Biological Activity: The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg.

Solubility: It is recommended to reconstitute the lyophilized EPO-alpha in sterile 18MOmegacm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.

References: 1.Title:Zinc Transporters ZnT1 (Slc30a1), Zip8 (Slc39a8), and Zip10 (Slc39a10) in Mouse Red Blood Cells Are Differentially Regulated during Erythroid Development and by Dietary Zinc Deficiency1-3Publication:Journal of Nutrition, doi:10.3945/jn.108.093575Vol. 138, No. 11, 2076-2083, November 2008© 2008 American Society for Nutrition J. Nutr. 138:2076-2083, November 2008Link:http://jn.nutrition.org/content/138/11/2076.full2.Title:Systemically delivered Erythropoietin transiently enhances adult hippocampal neurogenesis.Publication:Article first published online: 7 MAY 2007 DOI:10.1111/j.1471-4159.2007.04684.x Journal of Neurochemistry Volume 102, Issue 6, pages 1953-1965, September 2007.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2007.04684.x/full3.Title:ZINC TRANSPORTER EXPRESSION IN MATURE RED BLOOD CELLS ANDDIFFERENTIATING ERYTHROID PROGENITOR CELLS.Publication:UNIVERSITY OF FLORIDA© 2007 Moon-Suhn RyuLink:http://ufdcimages.uflib.ufl.edu/UF/E0/02/14/46/00001/ryu_m.pdf4.Title:Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury.Publication:First Published Online May 3, 2012 doi: 10.5966/sctm.2011-0048 Stem Cells Trans Med May 2012 vol. 1 no. 5 373-383Link:http://stemcellstm.alphamedpress.org/content/1/5/373.full

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.