IFN a 2a Human
Product Name: IFN a 2a Human
Product No: CYT-204
Synonym(s): IFN-Alpha 2a Human Recombinant, Leukocyte IFN, B cell IFN, Type I IFN, IFNA2, IFN-a 2a.
One-Letter-Code: CDLPQTHSLGSRRTLMLLAQMRKISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEM IQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAV RKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE. N-terminal methionine has been completely removed enzymatically.
Description: IFN Alpha Human 2a Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The difference between IFNA2A and IFNA2B is in the amino acid present at position 23. IFN-alpha 2a has a lysine at that position 23 while IFN-alpha 2b has arginine.The IFNA2A gene was obtained from human leukocytes.The IFNA2A is purified by proprietary chromatographic techniques.
Protein Derived From: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97.0% as determined by both:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Stability: Lyophilized IFNA-2A although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IFN-alpha 2a should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 270,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized IFNA2A in sterile 18MOmegacm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.
Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IFN-a 2a as a Reference Standard.
References: 1.Title:Gender specificity of altered human immune cytokine profiles in aging.Publication:Published online before print May 7, 2010, doi: 10.1096/fj.10-160911 September 2010 The FASEB Journal vol. 24 no. 9 3580-3589 Link:http://www.fasebj.org/content/24/9/3580.full 2.Title: Differential involvement of 5-HT1A and 5-HT1B/1D receptor in human IFN a-induced immobilityPublication: Arzneimittel-Forschung 60.3 (2009): 109-115.Link: http://www.ijppp.org/files/IJPPP905002.pdf
Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.