IFN g Human
Product Name: IFN g Human
Product No: CYT-206
Synonym(s): IFN-Gamma Human Recombinant, Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.
One-Letter-Code: MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ.
Description: IFN-gamma Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 17kDa.The IFN-gamma is purified by proprietary chromatographic techniques.
Protein Derived From: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Stability: Lyophilized IFN gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IFN-gamma should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: The specific activity as determined in a viral resistance assay is < 0.05 ng/ml, corresponding to a specific activity of 2.0 x 10,000,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized IFN-gamma in sterile distilled water or 20mM AcOH not less than 100microg/ml, which can then be further diluted to other aqueous solutions.
Protein content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.640 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IFN-g as a Reference Standard.
References: 1.Title:Candida albicansabrogates the expression ofIFN-G-inducible protein-10 in human keratinocytes.Publication:Article first published online: 18 JUL 2008DOI: 10.1111/j.1574-695X.2008.00457.xLink:http://onlinelibrary.wiley.com/doi/10.1111/j.1574-695X.2008.00457.x/full 2.Title: Augments Cytokine-Induced Nitric Oxide Production in Human Alveolar Epithelial CellsPublications:Antimicrobial agents and chemotherapy 50.1 (2006): 396-398Link:http://aac.asm.org/content/50/1/396.full 3.Title:Selective Activation of Human Dendritic Cells by OM-85 through a NF-kB and MAPK Dependent PathwayPublications:PloS one 8.12 (2013): e82867Link:http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0082867#pone-0082867-g005 4.Title:A New Strategy to Induce Effective Antitumour Response In Vitro and In VivoPublications:Scandinavian journal of immunology68.3 (2008): 287-296Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2008.02140.x/full 5.Title:Capture of Tumor Cell Membranes by Trogocytosis Facilitates Detection and Isolation of Tumor-Specific Functional CTLsPublication:Cancer research68.6 (2008): 2006-2013.Link:http://cancerres.aacrjournals.org/content/68/6/2006.full 6.Title:Curcumin induces maturation-arrested dendritic cells that expand regulatory T cells in vitro and in vivoPublication:Clinical & Experimental Immunology 162.3 (2010): 460-473.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2249.2010.04232.x/full
Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.