Product Name: IGF1 Human

Product No: CYT-216

Synonym(s): IGF-1 Human Recombinant, Somatomedin C, IGF-I, IGFI, IGF1, IGF-IA MGF.

One-Letter-Code: GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA.

Description: IGF1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kDa.IGF-1 is purified by proprietary chromatographic techniques.

Protein Derived From: Escherichia Coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC(b) Analysis by SDS-PAGE.

Stability: Lyophilized IGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IGF1 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Biological Activity: The ED50 as determined by a cell proliferation assay using murine BALB/C 3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of> 1×106 units/mg.

Solubility: It is recommended to reconstitute the lyophilized IGF-1 in sterile 18MOmega-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Background: IGF stands for INS like growth factors, which are proteins that have a high similarity to INS. They are part of a complicated process that uses cells to communication with the physiologic environment around them. IGF’s complex system is often called the ‘axis’. This consists of:? Two cell-surface receptors (IGF1R and IGF2R)? Two ligands (IGF-1 and IGF-2) ? A family of six high-affinity IGF-binding proteins.? Proteases. The Effect IGF Has On The BodyMany unique tissue types express the IGF-1 receptor, and the effects can vary. It induces the survival of neurons, may catalyse skeletal muscle hypertrophy by inducing protein synthesis, and by blocking muscle atrophy. It works as a protector for cartilage cells, and may work to be an anabolic factor for the bones. When used in high concentrations, it can activate the INS receptor, and can even complement the effects that INS has on the body. Diseases And IGFDiseases and IGF are closely related, and a number of them can be affected. The INS IGF axis is thought to have an effect on aging, with an increased life span shown in fruit flies when used in studies. It is also important to note the crucial role that IGF plays in cancer and diabetes - IGF- 1 has been shown to stimulate growth in both prostate and breast cancer cells. The degree of risk that IGF-1 poses is up for debate, and many scientists are not in agreement. IGF has also shown to have the ability to decrease blood glucose levels, although not quite as effective as INS. How Was IGF Discovered? Investigators began studying the effects of biological substances on cells and tissues outside the body when IGFs were discovered. The name is self explanatory in the fact that IGF performs INS actions in some tissues, but is less potent than INS at decreasing blood sugar. Its fundamental action is to stimulate growth, whether that be within the epidermal growth factor or the nerve growth factor. What’s The Difference Between IGF-1 And IGF-2?The two types of IGF are IGF-1 and IGF-2. Although the names are similar, the specific actions that they take are different - they bind and activate completely difference receptors. The major actor in them both is the effect that they have on cell growth. Most of the actions of the pituitaryGH are mediated by IGFs, but predominantly IGF-1.GH works to stimulate many tissues within the body, especially the liver which then secretes IGF-1. This then causes hypertrophy, or in layman's terms, an increase in cell size, as well as hyperplasia which is an increase in the number of cells. The IGF-1 concentration will increase during childhood and hit peak during puberty, but will then decrease afterwards, as does the hormone secretion itself. It has been proven that children and adults with a deficiency of theGH have low serum IGF-1 concentrations when put in comparison with others in the same age range. Patients who have conditions like acromegaly have been shown to have increased serum IGF-1 concentrations. The production of IGF-2 is less dependent on the secretion ofGH than IGF-1, and is much less important for stimulating linear growth.

References: 1.Title:Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis. Publication:Journal of Endocrinology (2009) 202, 287-297. Link: http://joe.endocrinology-journals.org/content/202/2/287.full.pdf Application: support spermatogenesis in an androgen-independent manner (Leal et al. 2006)2.Title:Suppression of Anoikis by SKP2 Amplification and Overexpression Promotes Metastasis of Esophageal Squamous Cell Carcinoma .Publication:doi: 10.1158/1541-7786.MCR-08-0092 Mol Cancer Res January 2009 7; 12 Link:http://mcr.aacrjournals.org/content/7/1/12.full3.Title:MECHANISMS OF BIOMATERIAL MEDIATED FIBROTIC RESPONSES AND STRATEGIES TO IMPROVE TISSUE REACTIONS TO BIOMATERIAL IMPLANTS.Publication:THE UNIVERSITY OF TEXAS AT ARLINGTONMay 2010Link:http://dspace.uta.edu/bitstream/handle/10106/4918/Thevenot_uta_2502D_10646.pdf?sequence=14. Title: Cancer-Associated Carbonic Anhydrases IX and XII: Effect of Growth Factors on Gene Expression in Human Cancer Cell Lines.Publication: Journal of Cancer Molecules 5(3): 73-78, 2010. Link: http://mupnet.com/JOCM%205(3)%2073-78.pdf

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.