Product Name: EGF Human

Product No: CYT-217

Synonym(s): Epidermal Growth Factor Human Recombinant, Urogastrone, URG, EGF.

One-Letter-Code: NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.

Description: Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6.2kDa. The EGF is purified by proprietary chromatographic techniques.

Protein Derived From: Escherichia Coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 98.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.

Stability: Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Biological Activity: The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of >1.0x107 IU/mg.

Solubility: It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MOmega-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

References: 1.Title:Activation of the Hedgehog pathway in pilocytic astrocytomas.Publication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010 Link:http://neurooncology.oxfordjournals.org/content/12/8/790.full2.Title:Evaluation of bioactivity and effect of polymeric stabilizers during heat treatment for the unfolded fraction of human epidermal growth factor.Publication:SEN'I GAKKAISHI vol.67,nov.8(2011)Link:http://www.jstage.jst.go.jp/article/fiber/67/8/185/_pdf3.Title:GPR56 is essential for testis development and male fertility in mice.Publication:Article first published online: 27 OCT 2010 DOI: 10.1002/dvdy.22468Link:http://onlinelibrary.wiley.com/doi/10.1002/dvdy.22468/full4.Title:Changing the Receptor Specificity of Anthrax Toxin.Publication:doi: 10.1128/?mBio.00088-12 1 May 2012 mBio vol. 3 no. 3 e00088-12 Link:http://mbio.asm.org/content/3/3/e00088-12.full5.Title:Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins.Publication:Kallio H, Tolvanen M, J?nis J, Pan P-w, Laurila E, et al. (2011) Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. PLoS ONE 6(11): e27152. doi:10.1371/journal.pone.0027152Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00271526. Title: Cancer-Associated Carbonic Anhydrases IX and XII: Effect of Growth Factors on Gene Expression in Human Cancer Cell Lines.Publication: Journal of Cancer Molecules 5(3): 73-78, 2010. Link: http://mupnet.com/JOCM%205(3)%2073-78.pdf7.Title:EGR-1 activation by EGF inhibits MMP-9 expression and lymphoma growthPublication:Blood 116.5 (2010): 759-766.Link:http://www.bloodjournal.org/content/116/5/759.long?sso-checked=true

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.