Product Name: FGF 2 Human

Product No: CYT-218

Synonym(s): Fibroblast Growth Factor-Basic Human Recombinant, Prostatropin, FGF-basic, Basic FGF, HBGF-2, FGF-2, FGF-b.

One-Letter-Code: AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS.

Description: Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and having a molecular mass of 17.2kDa.The FGF-b is purified by proprietary chromatographic techniques.

Protein Derived From: Escherichia Coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 98.0% as determined by Analysis by SDS-PAGE.

Stability: Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine balb/c 3T3 cells is <0.1ng/ml, corresponding to a specific activity of graeter than 1.0x107 Units/mg.

Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18MOmega-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

References: 1.Title:Activation of the Hedgehog pathway in pilocytic astrocytomasPublication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010 Link:http://neurooncology.oxfordjournals.org/content/12/8/790.full2.Title:Phenotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation Publication:Advance Online Publication March 4, 2008 doi: 10.1242/?jcs.019315 April 1, 2008 J Cell Sci 121, 1002-1013. Link:http://jcs.biologists.org/content/121/7/1002.full3. Title:Embryonic Stem Cell-Derived Glial Precursors as a Vehicle for Sulfamidase Production in the MPS-IIIA Mouse Brain .Publication: Cell Transplantation ISSN: 0963-6897 DOI: 10.3727/096368910X498944 Volume 19, Issue 8, pages 985-998 Copyright © 2010 Cognizant Comm. CorpLink:http://www.ingentaconnect.com/content/cog/ct/2010/00000019/00000008/art000074.Title:Adult Human Dental Pulp Stem Cells Differentiate Toward Functionally Active Neurons Under Appropriate Environmental Cues.Publication: Article first published online: 22 MAY 2008 DOI:10.1634/stemcells.2007-0979 Copyright © 2008 AlphaMed Press.Link:http://onlinelibrary.wiley.com/doi/10.1634/stemcells.2007-0979/full5.Title: Successful implantation of physiologically functional bioengineered mouse internal anal sphincterPublication: American Journal of Physiology-Gastrointestinal and Liver Physiology 299.2 (2010): G430-G439.Link: http://ajpgi.physiology.org/content/299/2/G430

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.