GM CSF Human
Product Name: GM CSF Human
Product No: CYT-221
Synonym(s): Granulocyte Macrophage-Colony Stimulating Factor Human Recombinant, CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, MGC131935, MGC138897.
One-Letter-Code: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser.N-terminal methionine has been completely removed enzymatically.
Description: Granulocyte Macrophage Colony Stimulating Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. GM-CSF is purified by proprietary chromatographic techniques.
Protein Derived From: Escherichia Coli.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98.0% as determined by:1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE.
Stability: Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11,100,000 IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18MOmega-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Background: GM-CSF has similar effects to G-CSF which is widely used as a therapeutic agent in immunosupressed patients mostly to boost production of the myeloid lineage after chemotherapy-induced neutropenia. Using GM-CSF was also shown to boost anti-tumor immunity by promoting the maturation of antigen presenting dendritic cells (DCs) and Th1 responses. It was suggested for therapeutic uses in various cancers including follicular lymphoma, acute leukemia and prostate cancer. GM-CSF is particular affective as an antitumor vaccine when used in tumor cells engineered to secret GM-CSF. GM-CSF is also involved in the treatment of Crohn's disease. Another use of GM-CSF is as a vaccine adjuvant and it was also claimed to improve the general health condition of AIDS patients although this is a controversial issue and it was recently found that GM-CSF actually induces the expression of HIV-1in monocytes. GM-CSF was also found to be effective and is used in veterinary medicine. GM-CSF was also shown to play a pivotal role in regulation of embryo development and pregnancy and particularly in embryo implantation and placenta development. Abnormalities in GM-CSF production or receptor function were implicated in diseases such rheumatoid arthritis, juvenile and chronic myelomonocytic leukemia and alveolar proteinosis as well as in the pathogenesis of myeloproliferative diseases.
Protein content: GM-CSF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.963 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GEN computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of GM-CSF as a Reference Standard.
Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.