HGF Human, CHO

Product Name: HGF Human, CHO

Product No: CYT-251

Synonym(s): Hepatocyte Growth Factor Human Recombinant, CHO, Scatter Factor (SF), Hepatopoietin (HPTA), HGF.

Description: Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of approximately 75kDa. The HGF is purified by proprietary chromatographic techniques.

Protein Derived From: Chinese Hamster Ovarian Cells.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 97.0% as determined bySDS-PAGE.

Stability: Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution HGF should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Biological Activity: The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.

Solubility: It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18MOmegacm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.