BMP 7 Human, CHO
Product Name: BMP 7 Human, CHO
Product No: CYT-276
Synonym(s): Bone Morphogenetic Protein-7 Human Recombinant, CHO, Osteogenic Protein 1, BMP-7.
Description: N-TERMINAL---Human BMP-2 (Met 1 – Arg 282) Human BMP-7 (Ser 293 – Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the human rhBMP-7 mature chain (293~431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ~ 40kDa in SDS-PAGE under non-reducing conditions. BMP-7 is purified by proprietary chromatographic techniques.
Protein Derived From: Chinese Hamster Ovarian Cells.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Stability: Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP 7 Human should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Biological Activity: Measured in alkaline phosphatase activity assay using MC3T3-E1 cells. The ED50 for this effect is < 70ng/ml, corresponding to a Specific Activity of 14,286IU/mg.
Solubility: It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in 0.5mg/1ml sterile/ endotoxin free water.
References: 1.Title:Bone morphogenetic protein 7 induces mesenchymal-to-epithelial transition in melanoma cells, leading to inhibition of metastasis.Publication:Article first published online: 30 JUL 2009 DOI:10.1111/j.1349-7006.2009.01301.x © 2009 Japanese Cancer Association.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1349-7006.2009.01301.x/full2.Title:In Vivo Degradation of a Novel Poly(?-Caprolactone) Invertebral Cage for Anterior Cervical Discectomy and Fusion in a Porcine Model.Publication:Tissue Engineering and Regenerative Medicine International Society - EU Meeting -2010Galway, IrelandLink:http://22.214.171.124/docs/eu10_abstracts/TERMIS2010_0708.pdf3.Title:Pretreatment With Bone Morphogenetic Protein-7 (Bmp-7) Mimics Ischemia Preconditioning Following Intestinal Ischemia/Reperfusion Injury in the Intestine and Liver.Publication:November 2008 - Volume 30 - Issue 5 - pp 532-536 doi: 10.1097/SHK.0b013e31816f20f1 Basic Science Aspects.Link:http://journals.lww.com/shockjournal/Abstract/2008/11000/Pretreatment_With_Bone_Morphogenetic_Protein_7.8.aspx
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