Product Name: BMP 7 Human

Product No: CYT-333

Synonym(s): Bone Morphogenetic Protein-7 Human Recombinant, Osteogenic Protein 1, BMP-7.

One-Letter-Code: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys.

Description: Bone Morphogenetic Protein-7 Human Recombinant produced in E.Coli is a monomeric, non-glycosylated, polypeptide chain containing 139 amino acids and having a molecular mass of 15679.97 Dalton. The BMP-7 is purified by proprietary chromatographic techniques.

Protein Derived From: Escherichia Coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Stability: Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP 7 Human should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in 20mM-100mM acetic acid at a concentration of 0.1-0.5mg per ml. Stock solutions should be apportioned into working aliquots and stored at -20°C. Further dilutions should be made in appropriate buffered solutions.

References: 1.Title: Pulpal and periapical response of dogs' teeth after pulpotomy and use of recombinant human bone morphogenetic protein-7 as a capping agentPublications: J Dent Child 74 (2007): 79-84.Link: http://www.researchgate.net/profile/Francisco_Paula-Silva/publication/5374974_Pulpal_and_periapical_response_of_dogs'_teeth_after_pulpotomy_and_use_of_recombinant_human_bone_morphogenetic_protein-7_as_a_capping_agent/links/5413605e0cf2788c4b359755.pdf2.Title: Pretreatment with Bone Morphogenetic Protein-7 (BMP-7) Mimics Ischemia Preconditioning Following Intestinal Ischemia/Reperfusion Injury in The Intestine and LiverPublications: Shock (Augusta, Ga.)30.5 (2008): 532.Link:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3800102/3.Title: BMP-7 blocks mesenchymal conversion of mesothelial cells and prevents peritoneal damage induced by dialysis fluid exposurePublication: Nephrology Dialysis Transplantation(2010): gfp618.Link:http://ndt.oxfordjournals.org/content/early/2010/01/18/ndt.gfp618.full

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.