Product Name: IFN g Mouse

Product No: CYT-358

Synonym(s): IFN-Gamma Mouse Recombinant, Immune IFN, type II IFN, T cell IFN, MAF, IFNG, IFG, IFI, IFN-gamma.

One-Letter-Code: MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC.

Description: IFN-gamma Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kDa.The IFN-gamma is purified by proprietary chromatographic techniques.

Protein Derived From: Escherichia Coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Stability: Lyophilized IFN-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IFN-gamma should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Biological Activity: The specific activity as determined in a viral resistance assay is < 0.1 ng/ml, corresponding to a specific activity of 1 x 107 Units/mg.

Solubility: It is recommended to reconstitute the lyophilized IFN-gamma in sterile distilled water or 20mM AcOH at concentrations ranging between 0.1mg-0.5mg/ml, which can then be further diluted to other aqueous solutions.

References: Title:IFN-γ and interleukin-4 reciprocally regulate CD8 expression in CD8+ T cells.Publication:Published online before print November 6, 2008, doi: 10.1073/pnas.0809549105 PNAS November 11, 2008 vol. 105 no. 45 17475-17480 Link:http://www.pnas.org/content/105/45/17475.full

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.