Product Name: TGFB3 Human
Product No: CYT-368
Synonym(s): Transforming Growth Factor-Beta 3 Human Recombinant, Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
One-Letter-Code: MALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS.
Description: TGF-β 3 Human Recombinant produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing two 113 amino acid chains and having a total molecular mass of 25.8kDa. The TGF-β 3 is purified by standard chromatographic techniques.
Protein Derived From: Escherichia Coli.
Physical Appearance: Sterile Filtered clear solution.
Purity: Greater than 95.0% as determined by SDS-PAGE.
Stability: TGF-beta 3 although stable at room temperature for 1 week, should be stored at 4°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Biological Activity: The activity is determined by the ability to induce chondrogenic differentiation.
Protein content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard.
References: 1.Title: Altering the Architecture of Tissue Engineered Hypertrophic Cartilaginous Grafts Facilitates Vascularisation and Accelerates MineralisationPublication: DOI: 10.1371/journal.pone.0090716, March 04, 2014Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00907162.Title: Oxygen tension differentially regulates the functional properties of cartilaginous tissues engineered from infrapatellar fat pad derived MSCs and articular chondrocytesPublication: Osteoarthritis and Cartilage 18.10 (2010): 1345-1354.Link:http://www.sciencedirect.com/science/article/pii/S1063458410002293
Special Note(s): Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.