Product Name: IL 3 Mouse

Product No: CYT-371

Synonym(s): Interleukin-3 Mouse Recombinant, MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor, IL-3, MGC79398, MGC79399.

One-Letter-Code: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asp-Thr-His-Arg.

Description: Interleukin-3 mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 135 amino acids and having a molecular mass of 15100 Dalton. The IL-3 is purified by proprietary chromatographic techniques.

Protein Derived From: Escherichia Coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 97.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.

Stability: Lyophilized Interleukin-3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL-3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine M-NFS-60 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg.

Solubility: It is recommended to reconstitute the lyophilized Interleukin-3 in sterile 18MOmega-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Protein content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.154 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL3 as a Reference Standard.

References: 1.Title:Direct binding of Cbl to Y568 and Y936 of the stem cell factor receptor/c-Kit is required for ligand-induced ubiquitination, internalization and degradation.Publication:Biochemical Journal Immediate Publication. Published on 19 Jun 2006 as manuscript BJ20060464.Link:http://www.biochemj.org/bj/imps_x/pdf/BJ20060464.pdf2.Title:enhanced self -renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene sall4.Publication:Link:http://www.biomedcentral.com/content/pdf/1756-8722-4-38.pdf3.Title:Identification of Y589 and Y599 in the juxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase SHP2.Publication:Published online before printMay 9, 2006, doi:10.1182/blood-2005-07-008896Submitted: July 14, 2005Accepted: April 26, 2006 BloodSeptember 1, 2006vol. 108no. 51542-1550.Link:http://bloodjournal.hematologylibrary.org/content/108/5/1542.full

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.