PDGF BB Human

Product Name: PDGF BB Human

Product No: CYT-501

Synonym(s): Platelet-Derived Growth Factor BB Human Recombinant, Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide.

One-Letter-Code: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT.

Description: Platelet-Derived Growth Factor BB Human Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 2x109 amino acids (218 amino acids in total) and having a molecular mass of 24.3 kDa. PDGF-BB is purified by proprietary chromatographic techniques.

Protein Derived From: Escherichia Coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Stability: Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1.0-3.0 ng/ml.

Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile18MOmegacm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.

References: 1.Title:Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway.Publication:Published online before print October 30, 2009, doi: 10.1182/blood-2009-06-223719 Blood January 7, 2010 vol. 115 no. 1 51-60 .Link:http://bloodjournal.hematologylibrary.org/content/115/1/51.full2.Title: attenuates skeletal muscle dystrophy in mdx mice.Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548.Link:http://www.fasebj.org/content/23/8/2539.full 3.Title:Human Cord Blood-Derived Endothelial Progenitor Cells and Their Conditioned Media Exhibit Therapeutic Equivalence for Diabetic Wound Healing. Publication: Cell Transplantation, Vol. 19, pp. 1635–1644, 2010 0963-6897/10 $90.00 + .00 Printed in the USA. All rights reserved. DOI: 10.3727/096368910X516637 Copyright ? 2010 Cognizant Comm. Corp. Link:http://docserver.ingentaconnect.com/deliver/connect/cog/09636897/v19n12/s11.pdf?expires=1370351771&id=74450861&titleid=5476&accname=Guest+User&checksum=14BACCDE2ACF33

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.