Product Name: IL 21 Mouse

Product No: CYT-684

Synonym(s): Interleukin-21 Mouse Recombinant, Interleukin-21, IL-21, Il21.

One-Letter-Code: The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Ser-Ser.

Description: Interleukin-21 Mouse Recombinant produced in E.Coli is a single, non-glycosilated polypeptide chain containing 130 amino acids and having a total molecular mass of 15kDa. The Murine IL-21 is purified by proprietary chromatographic techniques.

Protein Derived From: Escherichia Coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Stability: Lyophilized Mouse IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse IL21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Biological Activity: The ED50=10 -50 ng/ml, determined by the cell proliferation assay of modified Ba/F3 cells.

Solubility: It is recommended to reconstitute the lyophilized Mouse IL21 in sterile 18MOmega-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.