TGFB1 Human Recombinant

Product Name: TGFB1 Human Recombinant

Product No: CYT-716

Synonym(s): Transforming Growth Factor-Beta 1 Human Recombinant, Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

One-Letter-Code: ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.

Description: TGFB1 Human Recombinant produced in CHO cells is a glycosylated homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25.6kDa. The TGFB1 is purified by proprietary chromatographic techniques.

Protein Derived From: CHO cells.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 95.0% as determined by SDS-PAGE.

Stability: Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Biological Activity: The ED50 as determined by the dose-dependent inhibition of IL-4-induced proliferation of HT-2 cells is 0.148ng/ml, corresponding to a specific activity of 6.8x106units/mg.

Solubility: It is recommended to reconstitute the lyophilized TGFB1 in sterile 10mM HClat a concentration of 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

References: 1.Title: attenuates skeletal muscle dystrophy in mdx mice .Publication:Published online before print March 16, 2009, doi: 10.1096/fj.09-129833 August 2009 The FASEB Journal vol. 23 no. 8 2539-2548 .Link:http://www.fasebj.org/content/23/8/2539.full2.Title:Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins.Publication:Kallio H, Tolvanen M, J?nis J, Pan P-w, Laurila E, et al. (2011) Characterization of Non-Specific Cytotoxic Cell Receptor Protein 1: A New Member of the Lectin-Type Subfamily of F-Box Proteins. PLoS ONE 6(11): e27152. doi:10.1371/journal.pone.0027152Link:http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027152 3.Title: Cancer-Associated Carbonic Anhydrases IX and XII: Effect of Growth Factors on Gene Expression in Human Cancer Cell Lines. Publication: Journal of Cancer Molecules 5(3): 73-78, 2010. Link: http://mupnet.com/JOCM%205(3)%2073-78.pdf

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.