ACE2 (18-740) Human, Fc

Product Name ACE2 (18-740) Human, Fc

Product Number ENZ-1126

Synonym(s) Angiotensin Converting Enzyme 2 (18-740 a.a.), Fc Human Recombinant

ACE-2 (Angiotensin converting enzyme 2) is an enzyme bound to cell membranes in various organs such as intestines arteries , lungs, heart, and kidney. It is an entry receptor of SARS coronaviruses as well as SARS-CoV-2. The coronavirus spike (S) glycoprotein is a class I viral fusion antigen located on the external envelope of the virion that takes part in a critical part in viral infection by identifying host cell receptors and facilitating fusion of the viral and cellular membranes. Two main domains in coronavirus S1 have been recognized, the N-terminal domain and C-terminal domain. One or the other and/or both S1 domains function as a receptor-binding domain.  SARS-CoV and MERS-CoV equally use C-domain to attach their receptors. ACE2 is a type I transmembrane antigen with an extracellular N-terminal domain having the catalytic site and an intracellular C-terminal tail.  ACE2 obtains a signal peptide, a transmembrane domain, and a single metalloproteinase active site containing an HEXXH zinc-binding domain. ACE-2 plays a role as a mono-carboxypeptidase which degrades Ang I to produce the nonapeptide Ang 1–9 and Ang II to create the heptapeptide Ang 1–7.

Description The HEK293 derived ACE2 Human recombinant protein contains the amino acids 18-740 fused to Fc tag at C-terminal. ACE2 Protein binds to SARS Coronavirus-2 [ CoV-2019 ] Spike receptor binding domain.

Purification Method Purified by Protein-G chromatographic technique.

Physical Appearance Sterile Filtered colorless solution.

Formulation ACE2 Human protein solution is supplied in 50mM Tris-HCl, pH7.5, and 150mM NaCl and glycerol.

Stability ACE-2 Human Recombinant Protein is shipped on ice packs. Upon arrival, Store at -20°C.

Biological Activity ACE2 activity was measured by its binding ability in a functional ELISA. The immobilized Recombinant Human ACE2 protein binds to SARS CoV2 Spike protein Receptor Binding Domain at 2ug per ml.

Special Note(s) For research use ONLY. Not for use on humans. Ships with ice pack.

Document(s) SDS