Product Name: C5a Mouse

Product No: PRO-085

Synonym(s): Complement Component C5a Mouse Recombinant, Complement C5, Hemolytic complement, C5, Hc, He, C5a.

One-Letter-Code: The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Leu-His-Leu-Leu.

Description: C5a Mouse Recombinant produced in E.Coli is a single, non-glycosylated, Polypeptide chain containing 77 amino acids and having a molecular mass of 9kDa.The Mouse C5a is purified by proprietary chromatographic techniques.

Protein Derived From: Escherichia Coli.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Purity: Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Stability: Lyophilized Mouse C5a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse C5a should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Biological Activity: The ED50 of Recombinant Mouse C5a as determined by its ability to induce N-acetyl-b-D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells was 5-20ng/ml.

Solubility: It is recommended to reconstitute the lyophilized Mouse C5a in sterile 18MOmegacm H2O not less than 100microg/ml, which can then be further diluted to other aqueous solutions.

Special Note(s): ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.