SARS Spike (306-515)

Product Name SARS Spike (306-515)

Product Number SARS-046

Synonym(s) Coronavirus Spike Receptor Binding Domain (306-515 a.a.), Recombinant; Spike glycoprotein; S glycoprotein; Peplomer protein; E2 glycoprotein precursor; Severe acute repiratory Syndrome-related Coronavirus; SARS; SRAS-CoV; SARS-CoV1; E2

One-Letter Code DGSMRVVPSG DVVRFPNITN LCPFGEVFNA TKFPSVYAWE RKKISNCVAD YSVLYNSTFF STFKCYGVSA TKLNDLCFSN VYADSFVVKG DDVRQIAPGQ TGVIADYNYK LPDDFMGCVL AWNTRNIDAT STGNYNYKYR YLRHGKLRPF ERDISNVPFS PDGKPCTPPA LNCYWPLNDY GFYTTTGIGY QPYRVVVLSF ELLNAPATVC GPKLHHHHHH

Introduction SARS Coronavirus is an enveloped virus containing 3 outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein takes part in virus infection cycle and is the primary target of neutralizing antibodies.

Description The recombinant SARS Spike containing a total of 220 amino acids (306-515) and having a calculated M.W. of 24.8 kDa.
SARS Spike  is fused to a 6 amino acid His-tag at C-terminus, and is purified by proprietary chromatographic techniques.

Source HEK293

Formulation
The SARS Spike (306-515) solution (0.5mg/ml) contains Phosphate-Buffered Saline (pH 7.4) and 10% Glycerol.

Stability Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.

Physical Appearance Sterile filtered clear solution.


Purity 
Protein is >95% pure as determined SDS-PAGE.

Storage Conditions -20ºC

Delivery Details 7-10 days

Special Note(s) For research use ONLY. Not for use on humans.

Document(s) SDS